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Description Single isomers of ROX are increasingly preferred for labeling peptides and nucleotides because they give better resolution in HPLC purifications that are often required in bioconjugations. Variable ratios of the 5- and 6-isomers often cause complications in the interpretation of labeling results and assay performances. 5-ROX is predominantly used to label peptides and proteins. Compared to other rhodamines, ROX is very unstable. Cautions must be exercised to prevent the deterioration during storage. [1]A brief survey of methods for preparing protein conjugates with dyes, haptens, and cross-linking reagents. Brinkley M,Bioconjug Chem (1992) 3:2-13 [2]Actomyosin-based retrograde flow of microtubules in the lamella of migrating epithelial cells influences microtubule dynamic instability and turnover and is associated with microtubule breakage and treadmilling. Waterman-Storer CM, Salmon ED,J Cell Biol (1997) 139:417-434 [3]Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations. Hung SC, Mathies RA, Glazer AN,Anal Biochem (1998) 255:32-38 |
