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Description 5-FAM SE is a single isomer, it is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. It has also been used to prepare various small fluorescent molecules. [1] Labeling and application by using 5-FITC, 6-FITC and mixture of 5- and 6- isomers a)H. Rinderknecht, Nature 1962, 193, 167. b)K. Muramoto, H. Meguro, K. Tuzimura, Agric. Biol. Chem. 1977, 41, 2059. [2] Labeling and application by using 5-FAM and 6-FAM a)S. Onoue, B. Liu, Y. Nemoto, M. Hirose, T. Yajima, Anal. Sci. 2006, 22, 1531. b)P. Theisen, C. McCullum, K. Upadhya, K. Jacobson, H. Vu, A. Andrus, Tetrahedron Lett. 1992, 33, 5033. [3] Labeling and application by using 5-carboxyfluorescein N-succinimidyl ester a)P. Breeuwer, J. Drocourt, F. M. Rombouts, T. Abee, Appl. Environ. Microbiol. 1996, 62, 178. b)D. A. Fulcher, S. W. J. Wong, Immunol. Cell Biol. 1999, 77, 559. [4] Labeling and application by using fuorescein-5-maleimide T. Cihlar, E. S. Ho, Anal. Biochem. 2000, 283, 49. [5] Labeling and application by using 5-aminofluorescein and 6-aminofluorescein W. B. Dandliker, A. N. Hicks, S. A. Levison, R. J. Brawn, Biochem. Biophys. Res. Commun. 1977, 74, 538. |
