产品分类PRODUCT CENTER
联系我们CONTACT US
全国服务热线：0571-86691505
传真：0571-86691509
邮箱：sales@hzhandybio.com
地址：浙江省杭州市滨江区伟业路高新软件园
网址：www.zjhandybio.com
 |
|
| 产品参数： | |
Description Xanthene fluorescent dye tetramethylrhodamine (TMR, TAMRA, , λexcitation=550 nm, λemission=573 nm) is extremely popular for covalent labeling of various types of biomolecules, especially oligonucleotides for various formats of DNA analysis.5- and 6-TAMRA isomers on oligonucleotides showed similar absorption and emission spectra.TAMRA, is the most robust of the 4 common rhodamine fluorophores for in vivo optical imaging of ovarian cancer metastases to the peritoneum.We next inserted a 5(6)-carboxytetramethylrhodamine (TAMRA) fluorophore flanking the lipid linkage and immediately preceding the peptide portion of the pepducin to allow for visualization in cell-imaging and in-gel fluorescence studies.Fluorescence microscopy and flow cytometry showed that PPCs, but not the neutral cisplatin or oxaliplatin, blocked the cellular entry of TAMRA-R9 (a nonarginine peptide, R9) coupled to the TAMRA fluorescent label 5-(and 6-)carboxytetramethylrhodamine) in Chinese hamster ovary (CHO), human colon carcinoma (HCT116), and osteosarcoma (SAOS-2) cells.We demonstrate here a robust luminescence resonance energy transfer (LRET)-based protein kinase assay by using NaYF4:Yb,Er, one of the most efficient upconversion nanophosphors (UCNPs), as an autofluorescence-free LRET donor and a tetramethylrhodamine (TAMRA)-labeled substrate peptide as the acceptor. [1]Anal. Chem., 2014, 86 (12), pp 6095–6102. [2]Mol. Pharmaceutics, 2012, 9 (6), pp 1795–1802. [3]Bioconjugate Chem., 2010, 21 (1), pp 64–73. [4]Bioconjugate Chem., 2008, 19 (8), pp 1735–1742. [5]Biochemistry, 2000, 39 (47), pp 14487–14494. |
