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Description Application: 5(6)-Carboxy-X-rhodamine may be used as a fluorescent dye in nucleic acid analytical research applications. ROX dyes are strongly red fluorescent. They have longer excitation and emission wavelengths than the 'conventional' rhodamines. These dyes are used to label peptides, proteins, and other biological ligands. 5-ROX, 6-ROX and their mixture 5(6)-ROX are used to label biomolecules by EDC-mediated reactions. The ratio of 6-isomer/5-isomer is in the range 3/1 to 6/1. Compared to other rhodamines, ROX is very unstable. Cautions must be exercised to prevent the deterioration during storage. [1]Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides Authors: Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J. Journal: Proc Natl Acad Sci U S A (2005): 5926 [2]Adsorption of oligonucleotides on PMMA/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence Authors: Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C. Journal: Langmuir (2004): 6834 [3]Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis Authors: Faulds K, Smith WE, Graham D. Journal: Anal Chem (2004): 412 [4]Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry Authors: Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J. Journal: Proc Natl Acad Sci U S A (2004): 5488 [5]Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport Authors: Yang JH, Basinger SF, Gross RL, Wu SM. Journal: Invest Ophthalmol Vis Sci (2003): 1312 [6]HLA-DRB fluorotyping by dark quenching and automated analysis Authors: Slateva K, Elsner HA, Albis-Camps M, Blasczyk R. Journal: Tissue Antigens (2001): 250 [7]Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction--amplified short tandem repeats during denaturing capillary electrophoresis Authors: Hahn M, Wilhelm J, Pingoud A. Journal: Electrophoresis (2001): 2691 [8]Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes Authors: Li Y, Glazer AN. Journal: Bioconjug Chem (1999): 241 [9]Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations Authors: Hung SC, Mathies RA, Glazer AN. Journal: Anal Biochem (1998): 32 [10]Differential display with carboxy-X-rhodamine-labeled primers and the selection of differentially amplified cDNA fragments without cloning Authors: Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I. Journal: Anal Biochem (1998): 82 |
