5(6)-Carboxy-X-rhodamine may be used as a fluorescent dye in nucleic acid analytical research applications.
ROX dyes are strongly red fluorescent. They have longer excitation and emission wavelengths than the 'conventional' rhodamines. These dyes are used to label peptides, proteins, and other biological ligands. 5-ROX, 6-ROX and their mixture 5(6)-ROX are used to label biomolecules by EDC-mediated reactions. The ratio of 6-isomer/5-isomer is in the range 3/1 to 6/1. Compared to other rhodamines, ROX is very unstable. Cautions must be exercised to prevent the deterioration during storage.
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