Single isomers of ROX are increasingly preferred for labeling peptides and nucleotides because they give better resolution in HPLC purifications that are often required in bioconjugations. Variable ratios of the 5- and 6-isomers often cause complications in the interpretation of labeling results and assay performances. 5-ROX is predominantly used to label peptides and proteins. Compared to other rhodamines, ROX is very unstable. Cautions must be exercised to prevent the deterioration during storage.
A brief survey of methods for preparing protein conjugates with dyes, haptens, and cross-linking reagents.
Brinkley M,Bioconjug Chem (1992) 3:2-13
Actomyosin-based retrograde flow of microtubules in the lamella of migrating epithelial cells influences microtubule dynamic instability and turnover and is associated with microtubule breakage and treadmilling.
Waterman-Storer CM, Salmon ED,J Cell Biol (1997) 139:417-434
Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations.
Hung SC, Mathies RA, Glazer AN,Anal Biochem (1998) 255:32-38