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Description 7-amino-4-methylcoumarin-3-acetic-acid (AMCA) conjugated to a secondary antiserum as a label for fluorescence immunohistochemistry.AMCA is now commercially available as a streptavidin conjugate. Its excitation wavelength is optimal at 351/364 nm with a UV argon ion laser and it emits optimally in the blue at 450 nm. AMCA-conjugated streptavidin yields a fluorescence intensity comparable to that obtained with FITC-streptavidin. The emission spectrum of AMCA does not interfere with that of FITC and phycoerythrin and thus permits triple color analysis of cell surface antigens without requiring the need for important electronic compensation. [1]Journal of Immunological Methods, Volume 128, Issue 1, 1990, Pages 39-49. [2]Neuroscience Letters, Volume 82, Issue 2, 23 November 1987, Pages 121-126. |
